Main sol. 1236
Internal Solution-ID: S2458
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.75 | g | 0.739 | 5.43 | |
| K2HPO4 | 1.50 | g | 1.478 | 8.485 | |
| NaCl | 0.90 | g | 0.887 | 15.173 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.197 | 0.969 | |
| NH4Cl | 0.90 | g | 0.887 | 16.577 | |
| Modified Wolin's mineral solution | 9.00 | ml | - | - | |
|
Tryptone
(BD Bacto) |
2.00 | g | 1.97 | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 1.97 | - | |
| FeSO4 x 7 H2O solution (0.1% w/v) | 0.50 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
| Sodium citrate | 2.00 | g | 1.97 | 7.635 | |
| Wolin's vitamin solution | 5.00 | ml | - | - | |
| Na2S x 9 H2O | 0.30 | g | 0.296 | 1.231 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except ferrous sulfate, citrate, vitamins and sulfide), adjust pH to 6.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add ferrous sulfate and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add citrate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and adjust pH of complete medium to 7.0, if necessary. | |||||
Media with this solution
| 1236 | SPOROLITUUS MEDIUM |