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Main sol. 591

Internal Solution-ID: S2458

Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.33 6.163
2 0.33 g 0.33 2.97
3 0.33 g 0.33 3.463
4 0.33 g 0.33 4.422
5 0.33 g 0.33 2.423
6 , adjust if required 175.00 g 174.825 2991.4
7 1.50 g 1.499 17.838
8 0.50 g 0.5 2.08
9 5.00 g 4.995 27.726
10
(Bacto Proteose Peptone No.3)
5.00 g 4.995 -
11 Trace element solution SL-10 1.00 ml - -
12 Vitamin solution 10.00 ml - -
13 2.00 mg 0.002 0.009
14 990.00 ml 987.238 54800
15 , might need strain adjustment
(Oxoid)
0.50 g/l - -
Instructions:
  1. Prepare the medium without NaHCO3, Na2S, glucose and vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Glucose (filter-sterilized and stored under nitrogen), vitamins (filter sterilized and stored under nitrogen), and sodium sulphide (autoclaved under nitrogen) are added from sterile stock solutions. The pH should be pH 7.5.
591 HALOBACTEROIDES/HALOINCOLA MEDIUM