Main sol. 1238
Internal Solution-ID: S2460
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
30.0 | g | 30 | - | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| Distilled water | 1000.0 | ml | - | - | |
| PYE | 20.0 | ml | - | - | |
|
Glucose
(50 %) |
2.0 | ml | 1 | 5.551 | |
|
Riboflavin
(filter-sterilized, 0.2 mg/ml) |
5.0 | ml | - | - | |
| 1 After autoclaving and cooling the medium, 20 ml 50 x PYE (100 g peptone and 50 g yeast extract in 1l distilled water, autoclaved), 2 ml 50 % glucose (autoclaved) and 5 ml filter-sterilized riboflavin (0.2 mg ml-1) were added. | |||||
Media with this solution
| 1238 | WOODSHOLEA MEDIUM |