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Main sol. 142a

Internal Solution-ID: S247

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.00 g 15 256.674
(NH4)2SO4 1.00 g 1 7.568
MgSO4 x 7 H2O 1.50 g 1.5 6.086
CaCl2 x 2 H2O 0.42 g 0.42 2.857
Trace element solution SL-10 1.00 ml - -
Bromothymol blue
(0.1% w/v)
4.00 ml 0.004 0.006
K2HPO4 0.50 g 0.5 2.871
Na2S2O3 x 5 H2O 5.00 g 5 20.146
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust pH of the complete medium to 7.6 with a sterile 5% (w/v) Na2CO3 solution.
142a THIOMICROSPIRA PSYCHROPHILA MEDIUM