Main sol. 142a
Internal Solution-ID: S247
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.00 | g | 15 | 256.674 | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| MgSO4 x 7 H2O | 1.50 | g | 1.5 | 6.086 | |
| CaCl2 x 2 H2O | 0.42 | g | 0.42 | 2.857 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Bromothymol blue
(0.1% w/v) |
4.00 | ml | 0.004 | 0.006 | |
| K2HPO4 | 0.50 | g | 0.5 | 2.871 | |
| Na2S2O3 x 5 H2O | 5.00 | g | 5 | 20.146 | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust pH of the complete medium to 7.6 with a sterile 5% (w/v) Na2CO3 solution. | |||||
Media with this solution
| 142a | THIOMICRORHABDUS MEDIUM |