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Main sol. 143

Internal Solution-ID: S248

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Crotonic acid	6.00	g	6	69.695
NaOH	0.30	g	0.3	7.501
(NH₄)₂HPO₄	0.15	g	0.15	1.136
K₂HPO₄	0.10	g	0.1	0.574
MgCl₂ x 6 H₂O	0.03	g	0.03	0.148
CaCl₂ x 2 H₂O	0.04	g	0.04	0.272
NH₄Cl	0.05	g	0.05	0.935
MgSO₄ x 7 H₂O (0.1% w/v)	0.60	ml	6.0e-4	0.002
MnSO₄ x 2 H₂O (0.1% w/v)	0.40	ml	4.0e-4	0.002
FeSO₄ x 7 H₂O (0.1% w/v in 0.1 N H₂SO₄)	0.40	ml	4.0e-4	0.001
(NH₄)₆Mo₇O₂₄ x 4 H₂O	10.00	mg	0.01	-
Biotin (0.1% w/v)	0.04	ml	4.0e-5	1.6e-4
p-Aminobenzoic acid (0.1% w/v)	0.80	ml	8.0e-4	0.006
Tryptone (BD BBL)	1.00	g	1	14.069
Yeast extract (OXOID)	1.00	g	1	-
Sodium resazurin (0.1% w/v)	0.50	ml	5.0e-4	0.002
K₂CO₃	4.00	g	4	28.942
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients (except potassium carbonate) and sparge medium with 100% N₂ gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. An anoxic stock solution of the potassium carbonate is autoclaved separately under 80% N₂ and 20% CO₂ gas mixture and thereafter added to the sterile medium. Adjust pH of the complete medium to 6.8 - 7.0. Prior to inoculation add 10 - 20 mg/l sodium dithionite from a 5% (w/v) stock solution that has been freshly prepared under 100% N₂ gas and sterilized by filtration to reduce the medium.

Compound

Amount

Unit

Conc. [g/L]

Conc. [mM]

Crotonic acid

6.00

69.695

NaOH

0.30

0.3

7.501

(NH₄)₂HPO₄

0.15

1.136

K₂HPO₄

0.10

0.1

0.574

MgCl₂ x 6 H₂O

0.03

0.148

CaCl₂ x 2 H₂O

0.04

0.272

NH₄Cl

0.05

0.935

MgSO₄ x 7 H₂O
(0.1% w/v)

0.60

6.0e-4

0.002

MnSO₄ x 2 H₂O
(0.1% w/v)

0.40

4.0e-4

0.002

FeSO₄ x 7 H₂O
(0.1% w/v in 0.1 N H₂SO₄)

0.40

4.0e-4

0.001

(NH₄)₆Mo₇O₂₄ x 4 H₂O

10.00

0.01

Biotin
(0.1% w/v)

0.04

4.0e-5

1.6e-4

p-Aminobenzoic acid
(0.1% w/v)

0.80

8.0e-4

0.006

Tryptone
(BD BBL)

1.00

14.069

Yeast extract
(OXOID)

1.00

Sodium resazurin
(0.1% w/v)

0.50

5.0e-4

0.002

K₂CO₃

4.00

28.942

Distilled water

1000.00

1 Dissolve ingredients (except potassium carbonate) and sparge medium with 100% N₂ gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. An anoxic stock solution of the potassium carbonate is autoclaved separately under 80% N₂ and 20% CO₂ gas mixture and thereafter added to the sterile medium. Adjust pH of the complete medium to 6.8 - 7.0. Prior to inoculation add 10 - 20 mg/l sodium dithionite from a 5% (w/v) stock solution that has been freshly prepared under 100% N₂ gas and sterilized by filtration to reduce the medium.

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Main sol. 143

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Main sol. 143

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