Main sol. 1251
Internal Solution-ID: S2483
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| NaNO3 | 1.5 | g | 1.5 | 17.648 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.5 | 2.459 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 1 | - | |
| Na2CO3 | 1.0 | g | 1 | 9.435 | |
| D-Glucose | 3.5 | g | 3.5 | 19.427 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate and glucose), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add glucose from a sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be 7.0. | |||||
Media with this solution
| 1251 | CALDINITRATIRUPTOR MEDIUM |