Main sol. 1263
Internal Solution-ID: S2497
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 30.00 | g | 30 | 513.347 | |
KCl | 0.20 | g | 0.2 | 2.683 | |
K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 2.76 | g | 2.76 | 26.041 | |
NaHCO3 | 24.00 | g | 24 | 285.69 | |
Yeast extract | 0.50 | g | 0.5 | - | |
Cellobiose | 5.00 | g | 5 | 14.607 | |
Wolin's vitamin solution | 2.00 | ml | - | - | |
Na2S x 9 H2O | 0.40 | g | 0.4 | 1.665 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, bicarbonate, yeast extract, cellobiose, vitamins and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate to the medium, dissolve and adjust to pH 9.4. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum bottles and autoclave. To the autoclaved medium add yeast extract, cellobiose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Stock solutions of vitamins and cellobiose should be sterilized by filtration. |
1263 | SPIROCHAETA DISSIPATITROPHA MEDIUM |