Back to all solutions

Ferrous sulfide sludge

Internal Solution-ID: S2526

This solution is used in 4 mediaand was first defined in medium 1267.

Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 15.4 g 154 553.927
Na2S x 9 H2O 12.3 g 123 512.112
Distilled water 100.0 ml - -
1 Heat distilled water to 50°C in a 250 ml beaker with a stir bar present. While rapidly stirring the water, add the ferrous sulfate followed immediately by the sodium sulfide. The formed black FeS sludge is decanted into a glass bottle that can be stoppered. The FeS is allowed to settle for several hours and then the overlying water is decanted and replaced. This procedure is repeated at least five times to wash the FeS. After washing, the pH of the FeS solution should be close to neutrality. The FeS suspension can be kept in closed bottles or tubes under a nitrogen atmosphere for at least three months.
1267a MARIPROFUNDUS (ES) MEDIUM
1267 FERRIPHASELUS (ES) MEDIUM
1267b GALLIONELLA (ES) MEDIUM
1597a METHANONATRONARCHAEUM MEDIUM