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Solution A

Internal Solution-ID: S2532

Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1.11 20.749
MgSO4 x 7 H2O 0.20 g 0.222 0.901
CaCl2 x 2 H2O 0.10 g 0.111 0.755
K2HPO4 0.05 g 0.055 0.319
Wolfe's mineral elixir 1.00 ml - -
NaHCO3
(for top layer)
0.50 g 0.555 6.606
Agarose
(for top layer, low melt)
1.50 g 1.665 2.64
Distilled water 900.00 ml - -
1267b GALLIONELLA (ES) MEDIUM