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Main sol. 1270

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Internal Solution-ID: S2536

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 6.0 g 6 44.09
K2HPO4 14.0 g 14 80.379
Trisodium citrate x 2 H2O 1.0 g 1 3.4
MgSO4 x 7 H2O 0.2 g 0.2 0.811
DL-Asparagine x H2O 0.8 g 0.8 6.055
L-Arginine x HCl 0.2 g 0.2 -
L-Glutamic acid 0.2 g 0.2 1.359
Glycine 0.2 g 0.2 2.664
L-Histidine 0.2 g 0.2 1.289
L-Tryptophan 0.2 g 0.2 0.979
L-Proline 0.2 g 0.2 1.737
(NH4)2SO4
(don't include in dry mix) 		2.0 g 2 15.136
Glucose
(added separately) 		10.0 g 10 55.506
Distilled water 1000.0 ml - -
Vitamin B12 40.0 µg 4.0e-5 3.0e-5
1 Adjust pH to 6.8 - 7.2.
2 Final concentration of vitamin B12: 40 µg/L.
1270 B12 - MEDIUM GUTTMAN