Main sol. 1272
Internal Solution-ID: S2539
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 0.5 | g | 0.5 | 4.718 | |
| NaHCO3 | 15.0 | g | 15 | 178.557 | |
| NaCl | 15.0 | g | 15 | 256.674 | |
| K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10. | |||||
| 2 After sterilization add: | |||||
| Trace element solution | 2.0 | ml/l | - | - | |
|
MgCl2 x 6 H2O
(200.0 g/l) |
1.0 | ml/l | 0.2 | 0.984 | |
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
Media with this solution
| 1272 | THIOALKALIVIBRIO JANNASCHI |