Main sol. 1278
Internal Solution-ID: S2558
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 20.00 | g | 19.782 | 338.508 | |
Na2SO4 | 4.00 | g | 3.956 | 27.855 | |
MgCl2 x 6 H2O | 4.30 | g | 4.253 | 20.921 | |
NH4Cl | 0.25 | g | 0.247 | 4.623 | |
KCl | 0.34 | g | 0.336 | 4.511 | |
CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.942 | |
KH2PO4 | 0.14 | g | 0.138 | 1.018 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Na-DL-lactate | 2.00 | g | 1.978 | 17.653 | |
Yeast extract
(OXOID) |
1.00 | g | 0.989 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
Na2CO3 | 1.00 | g | 0.989 | 9.332 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
DL-Dithiothreitol | 0.50 | g | 0.495 | 3.205 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, vitamins, and dithiothreitol), adjust pH to 6.8 - 7.0, and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add carbonate from a sterile anoxic stock solution prepared under an 80% N2 and 20% CO2 gas mixture. Add vitamins, and dithiothreitol from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Adjust the pH of the complete medium to 7.0, if necessary. |
1278 | ARCHAEOGLOBUS SULFATICALLIDUS MEDIUM |