Main sol. 1281
Internal Solution-ID: S2569
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Medium 1281 | 75.00 | ml | - | - | |
Salt Solution II | 75.00 | ml | - | - | |
Trypticase
(BBL) |
10.00 | g | 10 | - | |
Yeast extract | 5.00 | g | 5 | - | |
Glucose | 0.25 | g | 0.25 | 1.388 | |
Cellobiose | 0.25 | g | 0.25 | 0.73 | |
Maltose | 0.25 | g | 0.25 | 0.73 | |
Starch
(soluble) |
0.25 | g | 0.25 | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.3 | 1.708 | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
Vitamin solution | 1.00 | ml | - | - | |
Na2CO3
(8% w/v) |
2.50 | ml | 0.2 | 1.887 | |
Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
1 Boil 10 minutes under N2 stream, cool to room temperature. Adjust pH to 7.3 and autoclave for 20 minutes at 121°C. After autoclaving add sterile, anoxic solutions marked with #. For preparation of solid medium add 15.0 g agar per liter. |
1281 | ANAEROCELLA MEDIUM |