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Main sol. 1290

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Internal Solution-ID: S2584

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 950 ml - -
Solution B 25 ml - -
Solution C 1 ml - -
Solution D 25 ml - -
1 Dissolve ingredients of solution A, adjust pH to 8.0 with 1 N NaOH and boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense solution A under same gas atmosphere in anoxic culture vessels (e.g., Hungate-type tubes) and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of solutions B, C and D prepared under 100% N2 gas and sterilized by filtration. Adjust pH of the completed medium to 8.0 with a sterile anoxic solution of 0.1 N HCl or 0.1 N NaOH, if necessary.
1290 ALKALIPHILUS MEDIUM