Main sol. 1293
Internal Solution-ID: S2591
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 5.0 | g | 5 | 85.558 | |
| NaNO3 | 1.7 | g | 1.7 | 20.001 | |
|
Yeast extract
(OXOID) |
5.0 | g | 5 | - | |
|
Tryptone
(BD Bacto) |
10.0 | g | 10 | - | |
| NH4HCO3 | 3.2 | g | 3.2 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate) and adjust pH to 8.0, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add bicarbonate from a sterile anoxic 3.2% (w/v) stock solution prepared under 80% N2 and 20% CO2 gas mixture (e.g., add 1 ml stock solution per 9 ml medium). Adjust pH of the complete medium to 7.5 - 7.8, if necessary. | |||||
| 2 After inoculation add sterile 100% H2 gas to an overpressure of 0.5 bar. | |||||
Media with this solution
| 1293 | SYMBIOBACTERIUM THERMOPHILUM MEDIUM |