Main sol. 1299
Internal Solution-ID: S2597
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
30.00 | g | 29.94 | - | |
|
Yeast extract
(OXOID) |
0.10 | g | 0.1 | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 1.996 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| D-Glucose | 2.00 | g | 1.996 | 11.079 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.039 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except glucose, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add glucose, vitamins and sulfide from sterile anoxic stock solution prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 6.5 - 7.0, if necessary. | |||||
Media with this solution
| 1299 | THERMOSIPHO AFFECTUS MEDIUM |