Main sol. 1301
Internal Solution-ID: S2601
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.41 | g | 0.41 | 3.013 | |
Na2HPO4 | 0.43 | g | 0.43 | 3.029 | |
MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
CaCl2 x 2 H2O | 0.11 | g | 0.11 | 0.748 | |
NaCl | 0.30 | g | 0.3 | 5.133 | |
NH4Cl | 0.30 | g | 0.3 | 5.609 | |
Trace element solution | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Yeast extract | 0.20 | g | 0.2 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
D-Glucose | 1.80 | g | 1.8 | 9.991 | |
Wolin's vitamin solution | 2.00 | ml | - | - | |
Na2S x 9 H2O | 0.30 | g | 0.3 | 1.249 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, glucose, vitamins and reducing agents) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.0-7.2, if necessary. |
1301 | TEPIDANAEROBACTER ACETATOXYDANS MEDIUM |