Main sol. 1325
Internal Solution-ID: S2657
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 5.0 | g | 5 | - | |
| Peptone | 5.0 | g | 5 | - | |
| Glucose | 2.0 | g | 2 | 11.101 | |
| KH2PO4 | 1.0 | g | 1 | 7.348 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
|
Na2CO3
(7.5% w/v) |
40.0 | ml | 3 | 28.305 | |
| Trace element solution SL10 | 1.0 | ml | - | - | |
| Distilled water | 960.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15 | 44.599 | |
| 1 Final pH to 9.5. | |||||
| 2 Autoclave the Na2CO3 solution separately and combine with the rest of the medium after cooling both to about 50°C. Agar may be added at 15g/l to solidify the medium | |||||
Media with this solution
| 1325 | PARACOCCUS BOGORIENSIS MEDIUM |