Main sol. 152
Internal Solution-ID: S267
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nicotinic acid | 5.0 | g | 5 | 40.614 | |
Yeast extract | 10.0 | g | 10 | - | |
CaCl2 x 2 H2O | 8.0 | mg | 0.008 | 0.054 | |
MgSO4 x 7 H2O | 8.0 | mg | 0.008 | 0.032 | |
KH2PO4 | 40.0 | mg | 0.04 | 0.294 | |
K2HPO4 | 40.0 | mg | 0.04 | 0.23 | |
NaCl | 80.0 | mg | 0.08 | 1.369 | |
NaHCO3 | 0.4 | g | 0.4 | 4.762 | |
L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
Distilled water | 1000.0 | ml | - | - | |
1 Add nicotinic acid to the given amount of water, boil until dissolved and cool to room temperature. Add the other substances, adjust pH to 8.2 and distribute under 100% nitrogen atmosphere. |
152 | NICOTINIC ACID MEDIUM |