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Main sol. 152

Internal Solution-ID: S267

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nicotinic acid 5.0 g 5 40.614
Yeast extract 10.0 g 10 -
CaCl2 x 2 H2O 8.0 mg 0.008 0.054
MgSO4 x 7 H2O 8.0 mg 0.008 0.032
KH2PO4 40.0 mg 0.04 0.294
K2HPO4 40.0 mg 0.04 0.23
NaCl 80.0 mg 0.08 1.369
NaHCO3 0.4 g 0.4 4.762
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Distilled water 1000.0 ml - -
1 Add nicotinic acid to the given amount of water, boil until dissolved and cool to room temperature. Add the other substances, adjust pH to 8.2 and distribute under 100% nitrogen atmosphere.
152 NICOTINIC ACID MEDIUM