Main sol. 1337
Internal Solution-ID: S2678
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 24.0 | g | 24 | 410.678 | |
| MgCl2 x 6 H2O | 3.0 | g | 3 | 14.756 | |
| MgSO4 x 7 H2O | 2.0 | g | 2 | 8.114 | |
| KCl | 0.5 | g | 0.5 | 6.707 | |
| CaCl2 | 1.0 | g | 1 | 9.011 | |
| Distilled water | 1000.0 | ml | - | - | |
| Agar , for solid medium | 20.0 | g | 20 | 59.465 | |
| 1 Adjust to pH 8.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar. | |||||
| 2 After autoclaving cool down and add the following to 1 litre medium. Methanol and Vitamine B12 are filter sterilized. Solution T and Wolfe's Mineral Solution are separately autoclaved. Final pH 8.0. | |||||
| Methanol | 3.0 | ml | 2.376 | 74.153 | |
| Solution T | 20.0 | ml | - | - | |
| Wolfe's Mineral Solution | 10.0 | ml | - | - | |
| Vitamin B12 solution | 1.0 | ml | - | - | |
Media with this solution
| 1337 | METHYLOPHAGA MEDIUM |