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Wolfe's Mineral Solution
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Internal Solution-ID: S2680
Compound
Amount
Unit
Conc. [g/L]
Conc. [mM]
Nitrilotriacetic acid
1.50
g
1.5
7.848
MgSO
4
3.00
g
3
24.923
MnSO
4
0.50
g
0.5
3.311
NaCl
1.00
g
1
17.112
FeSO
4
0.10
g
0.1
0.658
CaCl
2
0.10
g
0.1
0.901
CoCl
2
0.10
g
0.1
0.77
ZnSO
4
0.10
g
0.1
0.62
CuSO
4
0.01
g
0.01
0.063
AlK(SO
4
)
2
0.01
g
0.01
0.039
H
3
BO
3
0.01
g
0.01
0.162
Na
2
MoO
4
0.01
g
0.01
0.049
Distilled water
1000.00
ml
-
-
1
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
2
Adjust to pH 8.0 with KOH and sterilize by autoclaving.
Media with this solution
1337
METHYLOPHAGA MEDIUM
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