Wolfe's Mineral Solution
Internal Solution-ID: S2680
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 | 3.00 | g | 3 | 24.923 | |
| MnSO4 | 0.50 | g | 0.5 | 3.311 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 | 0.10 | g | 0.1 | 0.658 | |
| CaCl2 | 0.10 | g | 0.1 | 0.901 | |
| CoCl2 | 0.10 | g | 0.1 | 0.77 | |
| ZnSO4 | 0.10 | g | 0.1 | 0.62 | |
| CuSO4 | 0.01 | g | 0.01 | 0.063 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 | 0.01 | g | 0.01 | 0.049 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. | |||||
| 2 Adjust to pH 8.0 with KOH and sterilize by autoclaving. | |||||
| 1337 | METHYLOPHAGA MEDIUM |