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Salt Water Solution (30% Stock Solution)

Internal Solution-ID: S2686

This solution is used in 3 media and was first defined in medium 1339.

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240 g 240 4106.78
KCl 7 g 7 93.897
MgCl2 x 6 H2O 30 g 30 147.563
MgSO4 x 7 H2O 35 g 35 142.002
CaCl2
(1 M)
5 ml 0.555 5
Distilled water 995 ml - -
1 Adjust to pH 7.5 with 2 M Tris-Base and sterilize by autoclaving.
1339 10% MGM-MEDIUM
1388 15% MGM-MEDIUM
1748 HALOFERAX GIBBONSII MEDIUM