Main sol. 1341
Internal Solution-ID: S2691
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MIN E - Base (10x) | 100 | ml | - | - | |
| Trace Metals (500x) | 2 | ml | - | - | |
| Distilled water | 900 | ml | - | - | |
| Phosphate buffer (10x) | 100 | ml | - | - | |
| Methanol | 20 | ml | 15.84 | 494.351 | |
|
Vitamin B12
(250 mg/l) |
1 | ml | - | - | |
| Agar , for solid medium | 15 | g | 15 | 44.599 | |
| 1 Add 100 ml of sterile phosphate buffer after autoclaving. | |||||
| 2 Autoclave the Mineral Salts Solution MIN E and phosphate buffer separately and combine after cooling. Add 20 ml/l filter sterilized methanol and 1 ml/l filter sterilized vitamin B12 (250 mg/l). The medium may be solidified by adding 15 g/l agar. | |||||
Media with this solution
| 1341 | MIN E - METHYLOVERSATILIS MEDIUM |