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Zeikus trace elements solution

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Internal Solution-ID: S2698

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.500 g 12.5 65.397
FeCl3 x 4 H2O 0.200 g 0.2 1.233
ZnCl2 0.100 g 0.1 0.734
MnCl2 x 4 H2O 0.100 g 0.1 0.505
H3BO3 0.010 g 0.01 0.162
CoCl2 x 6 H2O 0.017 g 0.017 0.131
CuCl2 x 2 H2O 0.020 g 0.02 0.117
CaCl2 x 2 H2O 0.100 g 0.1 0.68
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NaCl 1.000 g 1 17.112
Na2SeO3 0.020 g 0.02 0.116
Distilled water 1000.000 ml - -
1 Neutralise the nitrilotriacetic acid to a pH of 6.5 with KOH before adding the other trace elements.
1343 TISTLIA CONSOTENSIS MEDIUM