Main sol. 1344
Internal Solution-ID: S2699
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Taps | 17.4 | g | 17.4 | - | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Sodium acetate | 1.0 | g | 1 | 12.191 | |
| Disodium succinate | 1.0 | g | 1 | 6.171 | |
| Na2S2O3 x 5 H2O | 0.1 | g | 0.1 | 0.403 | |
| CaCl2 x 2 H2O | 75.0 | mg | 0.075 | 0.51 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| Na2-EDTA | 5.0 | mg | 0.005 | 0.015 | |
| Trace elements (A) | 1.0 | ml | - | - | |
| Neutralized sulfide solution (B) | 30.0 | ml | - | - | |
| Bicarbonate solution (C) | 30.0 | ml | - | - | |
| Vitamin solution (D) | 1.0 | ml | - | - | |
|
Vitamin B12
(0.01%) |
0.4 | ml | 4.0e-5 | 3.0e-5 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 8.3 | |||||
| 2 Mix ingredients vitamin B12, vitamin mixture, bicarbonate solution and neutralized sulfide solution. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers and autoclave at 121°C for 15 min. Separately autoclave neutralized sulfide solution under a N2 atmosphere. Sterilize Vitamin B12 solution, vitamin mixture, bicarbonate solution by filtration. Prior to inoculation, aseptically and anaerobically add vitamin B12 solution, vitamin mixture, bicarbonate solution and neutralized sulfide solution. | |||||
Media with this solution
| 1344 | LYU03 Medium |