Main sol. 1352
Internal Solution-ID: S2718
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.196 | 1.439 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.098 | 0.482 | |
| NH4Cl | 0.50 | g | 0.49 | 9.155 | |
| KCl | 0.20 | g | 0.196 | 2.628 | |
| NaCl | 60.00 | g | 58.766 | 1005.58 | |
| Na2CO3 | 68.30 | g | 66.895 | 631.158 | |
| NaHCO3 | 38.30 | g | 37.512 | 446.537 | |
| Trace element solution SL-4 | 1.00 | ml | - | - | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Yeast extract | 0.05 | g | 0.049 | - | |
|
Ethanol
(50% v/v) |
10.00 | ml | - | - | |
| Na2S x 9 H2O | 1.00 | g | 0.979 | 4.078 | |
| NaNO3 | 0.85 | g | 0.833 | 9.795 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 8.8- 9.5; gas atmosphere: 100% N2. | |||||
| 2 Dissolve ingredients except the sodium carbonate, sodium bicarbonate, ethanol, and sodium sulphide. Boil medium for a few minutes and cool to room temperature while flushing with N2. Add and dissolve sodium carbonate, sodium bicarbonate and sodium sulphide while gassing the head space only. Dispense and autoclave under N2. Before use add 10 ml/l (v/v) of 50% (v/v) ethanol solution (flushed and autoclaved under N2). | |||||
Media with this solution
| 1352 | FUCHSIELLA ALKALIACETIGENA MEDIUM |