Main sol. 1353
Internal Solution-ID: S2722
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 1.00 | g | 1 | - | |
| Tryptone | 5.00 | g | 5 | 70.343 | |
| Casamino acids | 5.00 | g | 5 | - | |
|
Beef extract
(Lab-Lemco-Powder) |
5.00 | g | 5 | - | |
| K2HPO4 | 1.00 | g | 1 | 5.741 | |
| NH4NO3 | 1.00 | g | 1 | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Cysteine HCl | 0.25 | g | 0.25 | 2.063 | |
| NiCl2 x 6 H2O | 7.50 | mg | 0.008 | 0.058 | |
| Trace element solution | 5.00 | ml | - | - | |
|
Resazurin
(0.1% v/v) |
0.50 | ml | - | - | |
| Artifical seawater solution | 250.00 | ml | - | - | |
| Distilled water | 750.00 | ml | - | - | |
| 1 Prepare the liquid medium without Na2S and cysteine-HCl. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Adjust to pH 7.0 with 10 M NaOH solution. Dispense the medium into Hungate tubes or serum bottles. Sterilize by autoclaving. Before inoculatiing add Na2S and cysteine-HCl from sterile stock solutions. | |||||
Media with this solution
| 1353 | YTBC - MEDIUM |