Main sol. 1359
Internal Solution-ID: S2738
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| NaCl | 0.40 | g | 0.4 | 6.845 | |
| NH4Cl | 0.68 | g | 0.68 | 12.713 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
| Na pyruvate | 0.50 | g | 0.5 | 4.544 | |
| Sodium succinate | 0.50 | g | 0.5 | 3.085 | |
| Na acetate | 0.50 | g | 0.5 | 6.095 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Ferric citrate
(0.1%) |
5.00 | ml | 0.005 | 0.02 | |
|
NaHCO3
(10%) |
15.00 | ml | 1.5 | 17.856 | |
|
Na2S x 9 H2O
(1M) |
1.00 | ml | 0.24 | 1 | |
|
Vitamin B12
(2mg/100ml) |
1.00 | ml | - | - | |
| Trace element solution SL7 | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the basal medium under nitrogen. | |||||
| 2 NaHCO3 solution (autoclaved in sealed, half full vessels under CO2), Na2S x 9H2O solution (autoclaved) and vitamin B12 solution (filter sterilised) is added to the autoclaved medium and the final pH adjusted to 6.6 - 7.4. | |||||
Media with this solution
| 1359 | PHAEOVIBRIO SULPHIDIPHILUS MEDIUM |