Back to all solutions

Mineral Salts Solution

Internal Solution-ID: S2750

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.05 g 0.05 -
Proteose peptone
(Difco no. 3)
0.05 g 0.05 -
Casamino acids 0.05 g 0.05 -
Glucose 0.05 g 0.05 0.278
Starch
(soluble)
0.05 g 0.05 -
MgSO4 x 7 H2O 5.00 mg 0.005 0.02
Agar 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Final pH 4.5 - 5.0; adjust with Alginic Acid before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
1365 1/10 R2A MEDIUM MODIFIED