Main sol. 1375
Internal Solution-ID: S2778
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.0 | g | 1 | 18.695 | |
MgCl2 x 6 H2O | 10.0 | g | 10 | 49.188 | |
MgSO4 x 7 H2O | 5.0 | g | 5 | 20.286 | |
KCl | 2.0 | g | 2 | 26.828 | |
K2HPO4 x 3 H2O | 0.5 | g | 0.5 | 2.871 | |
NaCl | 150.0 | g | 150 | 2566.74 | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
Trace element solution | 10.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
Glucose
(10% v/v) |
10.0 | ml | 15.44 | 85.702 | |
Na2CO3
(10% v/v) |
10.0 | ml | 25.3 | 238.706 | |
Na2S x 9 H2O
(2% v/v) |
10.0 | ml | - | - | |
Yeast extract
(10% v/v) |
10.0 | ml | - | - | |
L-Cysteine
(10% v/v) |
10.0 | ml | - | - | |
1 Final pH 8.3 | |||||
2 Prepare the medium without the Na2S, Na2CO3, NaHCO3, L-cysteine and substrates. Boil the medium and cool under a stream of nitrogen. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: |
1375 | HALANAEROBACTER JERIDENSE MEDIUM |