Main sol. 1378
Internal Solution-ID: S2796
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.000 | g | 25 | 427.789 | |
| MgCl2 x 6 H2O | 4.000 | g | 4 | 19.675 | |
| KCl | 0.500 | g | 0.5 | 6.707 | |
| CaCl2 x 2 H2O | 1.000 | g | 1 | 6.802 | |
| NH4Cl | 0.535 | g | 0.535 | 10.002 | |
| KH2PO4 | 0.100 | g | 0.1 | 0.735 | |
| Tryptone | 1.000 | g | 1 | 14.069 | |
| Glucose | 1.800 | g | 1.8 | 9.991 | |
| Sodium pyruvate | 0.550 | g | 0.55 | 4.998 | |
| Yeast extract | 1.000 | g | 1 | - | |
| NaHCO3 | 2.000 | g | 2 | 23.808 | |
| Cysteine-HCl | 0.300 | g | 0.3 | 1.903 | |
|
Resazurin
(1mg/ml) |
1.000 | ml | - | - | |
| Trace element solution | 1.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| Na2S | 0.030 | % | 0.3 | 3.795 | |
| Vitamin solution | 2.000 | ml | - | - | |
| 1 pH 7.0 | |||||
| 2 Prepare under N2/CO2 (80/20) gas. | |||||
| 3 Autoclave and add the following components after autoclaving from stock solutions: | |||||
| 4 sterile Na2S solution end concentration 0.03% | |||||
| 5 and filter-sterile Vitamin solution (see below) 0.2 ml / 100 ml. | |||||
Media with this solution
| 1378 | MO-CFXI MEDIUM |