Main sol. 1391
Internal Solution-ID: S2821
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 20.00 | g | 20 | 342.231 | |
K2HPO4 | 0.09 | g | 0.09 | 0.517 | |
KH2PO4 | 0.07 | g | 0.07 | 0.514 | |
CaCl2 x 2 H2O | 0.80 | g | 0.8 | 5.442 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
NaNO3 | 1.00 | g | 1 | 11.765 | |
MgSO4 x 7 H2O | 4.00 | g | 4 | 16.229 | |
MgCl2 x 6 H2O | 3.00 | g | 3 | 14.756 | |
KCl | 0.33 | g | 0.33 | 4.427 | |
FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.036 | |
Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
Fe(III) citrate | 0.01 | g | 0.01 | 0.041 | |
Yeast extract | 2.00 | g | 2 | - | |
Trace mineral solution | 5.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Prepare the medium under N2/CO2 (80%N2, 20%CO2) and fill in , the pH of the medium is adjusted with NaOH to 6.8. After autoclaving, the separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%H2, 20%CO2) is purged for 5 min. | |||||
2 Finally, the mix gas (77%H2, 20%CO2, 3%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm. | |||||
Wolin's vitamin solution | 1.00 | ml | - | - | |
NaHCO3 | 0.10 | % | 1 | 11.904 |
1391 | MMJHSY MEDIUM (modified) |