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Main sol. 793

Internal Solution-ID: S2829

Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.325 2.387
2 0.33 g 0.325 6.072
3 0.33 g 0.325 4.357
4 0.33 g 0.325 1.598
5 0.33 g 0.325 2.209
6 0.50 g 0.492 -
7
(87%)
3.00 ml 3.237 35.147
8 Trace element solution SL-10 1.00 ml - -
9 Selenite-tungstate solution 1.00 ml - -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 10.00 g 9.843 117.164
12 Vitamin solution 10.00 ml - -
13 0.50 g 0.492 2.049
14 1000.00 ml 982.487 54536.3
Instructions:
  1. Dissolve ingredients except bicarbonate, vitamins and sulfide, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dissolve bicarbonate and adjust pH to 6.7, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration.
793 MOORELLA GLYCERINI MEDIUM