Main sol. 165
Internal Solution-ID: S283
Equipment needed: Autoclave
Filter
Gassing station
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral solution 1 | 75.00 | ml | - | - | |
| Mineral solution 2 | 75.00 | ml | - | - | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| FeSO4 x 7 H2O solution (0.1% w/v) | 20.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.50 | g | 1.499 | 14.138 | |
| Cellobiose | 3.00 | g | 2.997 | 8.755 | |
|
Cellulose
(MN300) optional |
3.00 | g | 2.997 | - | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.422 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.04 | |
| Distilled water | 820.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, cellobiose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes and autoclave. Add cellobiose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Cellobiose and vitamins have to be sterilized by filtration. Prior to use adjust pH of complete medium to 7.2 - 7.4. Cellulose (MN 300, Whatman CF II, Kleenex tissue paper, or HCl treated cotton) can be used as an alternative to cellobiose. | |||||
Media with this solution
| 165 | ACETIVIBRIO CELLULOLYTICUS MEDIUM |