Main sol. 1404
Internal Solution-ID: S2849
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HEPES
(10M) |
2.38 | g | 2.38 | 9.987 | |
| NaCl | 30.00 | g | 30 | 513.347 | |
| MgSO4 x 7 H2O | 2.70 | g | 2.7 | 10.954 | |
| MgCl2 x 6 H2O | 4.30 | g | 4.3 | 21.151 | |
| NH4Cl | 0.25 | g | 0.25 | 4.674 | |
| KCl | 0.50 | g | 0.5 | 6.707 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| K2HPO4 x 3 H2O | 0.14 | g | 0.14 | 0.804 | |
| Peptone | 2.00 | g | 2 | - | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Trace Element Solution | 1.00 | ml | - | - | |
| Vitamin Solution | 10.00 | ml | - | - | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(2% v/v) |
1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium without the vitamin solution. Adjust to pH 7.25. Dispense the medium into Hungate tubes or serum bottles. Sterilize by autoclaving. Before inoculation add the sterile vitamin solution. | |||||
Media with this solution
| 1404 | MEDIUM FOR STRAIN 2M70-1 |