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Mineral Salts Solution for strain IT-4 and IT-9

Internal Solution-ID: S2873

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.881 511.302
KH2PO4 140.00 mg 0.139 1.025
CaCl2 x 2 H2O 800.00 mg 0.797 5.42
MgSO4 x 7 H2O 3.40 g 3.386 13.74
MgCl2 x 6 H2O 4.18 g 4.163 20.479
KCl 0.33 g 0.329 4.409
NH4Cl 0.25 g 0.249 4.655
NaNO3 0.25 g 0.249 2.93
Na2SiO3 x 9 H2O 0.20 mg 2.0e-4 7.0e-4
Fe2(SO4)3 x n H2O 20.00 mg 0.02 0.05
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Trace Element Solution 3.00 ml - -
Vitamin Solution 1.00 ml - -
Distilled water 1000.00 ml - -
1412 METHYLOTHERMUS MEDIUM