Main sol. 1413
Internal Solution-ID: S2877
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaHCO3 | 15.0 | g | 15 | 178.557 | |
| Na2CO3 | 95.0 | g | 95 | 896.328 | |
| NaCl | 16.0 | g | 16 | 273.785 | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the liquid medium. Dispense the medium into serum bottles. After sterilization pH 10.0. Before inoculation add from sterile stock solutions: | |||||
| MgSO4 | 1.0 | mM | 0.12 | 1 | |
| Yeast extract | 0.5 | g/l | 0.5 | - | |
|
Glucose
(20% v/v) |
2.0 | g/l | 2 | 11.101 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.0 | ml | - | - | |
Media with this solution
| 1413 | AMPHIBACILLUS MEDIUM |