Main sol. 1419
Internal Solution-ID: S2893
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral Salts Solution A | 50.0 | ml | - | - | |
| Mineral Salts Solution B | 1000.0 | ml | - | - | |
| 1 Autoclave solution A and B separately and combine after cooling. | |||||
| 2 Prepare the liquid medium. Before inoculation add from sterile stock solutions: | |||||
| Vitamin solution | 10.0 | ml | - | - | |
|
Vitamin B12
(0.1%) |
2.5 | µl | 2.5e-6 | 1.8e-6 | |
| 3 For solid media add 15.0 g agar to solution B | |||||
Media with this solution
| 1419 | HYPHOMICROBIUM MEDIUM |