Mineral Salts Solution B
Internal Solution-ID: S2895
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| Bacto peptone | 0.25 | g | 0.25 | - | |
| Trace Element Solution | 2.00 | ml | - | - | |
| Hutner's salts | 20.00 | ml | - | - | |
| Vitamin solution , add after autoclaving | 10.00 | ml | - | - | |
|
Vitamin B12
, add after autoclaving
(0.1%) |
2.50 | µl | 2.5e-6 | 1.8e-6 | |
| Distilled water | 1000.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| 1 Adjust pH to 7.5 | |||||
Media with this solution
| 1419 | HYPHOMICROBIUM MEDIUM |