Main sol. 1423
Internal Solution-ID: S2913
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KNO3 | 0.20 | g | 0.2 | 1.978 | |
KH2PO4 | 0.04 | g | 0.04 | 0.294 | |
MgSO4 x 7 H2O | 0.02 | g | 0.02 | 0.081 | |
CaCl2 x 2 H2O | 4.00 | mg | 0.004 | 0.027 | |
Trace elements Solution | 0.20 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 5.0. | |||||
2 Dispense the medium into serum bottles under gassing with CH4 (20% v/v). | |||||
3 Autoclave at 121 °C for 20 min. |
1423 | METHYLOSINUS MEDIUM MM2 |