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Main sol. 1425

Internal Solution-ID: S2916

Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.50 g 0.5 -
Yeast extract 0.25 g 0.25 -
Glucose 0.10 g 0.1 0.555
HEPES
(10 mM final concentration)
1.95 g 1.95 8.183
SSE (double concentrated) 500.00 ml - -
Trace element solution SL-10 1.00 ml - -
Distilled water 500.00 ml - -
1 Add to 1000 ml of medium after autoclaving:
Vitamin solution 1.00 ml - -
2 adjust pH to 7.0
1425 SSE/HD 1:10 MEDIUM (HEPES)