Main sol. 1425
Internal Solution-ID: S2916
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone | 0.50 | g | 0.5 | - | |
Yeast extract | 0.25 | g | 0.25 | - | |
Glucose | 0.10 | g | 0.1 | 0.555 | |
HEPES
(10 mM final concentration) |
1.95 | g | 1.95 | 8.183 | |
SSE (double concentrated) | 500.00 | ml | - | - | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Distilled water | 500.00 | ml | - | - | |
1 Add to 1000 ml of medium after autoclaving: | |||||
Vitamin solution | 1.00 | ml | - | - | |
2 adjust pH to 7.0 |
1425 | SSE/HD 1:10 MEDIUM (HEPES) |