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SSE (double concentrated)

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Internal Solution-ID: S2917

Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.2938 g 0.294 1.998
NH4Cl 0.1069 g 0.107 1.999
MgCl2 x 6 H2O 0.2036 g 0.204 1.001
(NH4)2SO4 0.1983 g 0.198 1.501
MgSO4 x 7 H2O 0.7390 g 0.739 2.998
CaSO4 x 2 H2O 0.8606 g 0.861 4.998
Ca(NO3)2 x 4 H2O 0.2360 g 0.236 0.999
Na NO3 0.4240 g 0.424 -
KH2PO4 0.0700 g 0.07 0.514
FeSO4 x 7 H2O 0.0111 g 0.011 0.04
K2SO4 0.0870 g 0.087 0.499
Distilled water 1000.0000 ml - -
1 Dissolve by shaking overnight. Sterilize by autoclaving.
1425 SSE/HD 1:10 MEDIUM (HEPES)