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Solution 1

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Internal Solution-ID: S2939

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.25 g 0.263 -
Yeast extract 0.25 g 0.263 -
CaCl2 x 2 H2O 0.10 g 0.105 0.716
MgCl2 x 6 H2O 0.10 g 0.105 0.518
Hutner's salts 20.00 ml - -
Tris-HCl buffer
(0.1M, pH 7.5) 		50.00 ml - 5
Agar 18.00 g 18.947 56.336
Distilled water 880.00 ml - -
1 Adjust pH to 7.5, autoclave at 121°C for 20 min.
1436 PIRELLULA MEDIUM (M31PY)