Main sol. 1442
Internal Solution-ID: S2964
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Gelatine | 2.00 | g | 2 | - | |
Yeast extract | 0.25 | g | 0.25 | - | |
Glucose | 0.25 | g | 0.25 | 1.388 | |
Hutner's salts | 20.00 | ml | - | - | |
Vitamin solution | 10.00 | ml | - | - | |
Tris-HCl buffer
(0.1M, bei Agar pH 8,5, pH 7.5) |
50.00 | ml | - | 5 | |
Artifical sea water | 250.00 | ml | - | - | |
Agar | 18.00 | g | 18 | 53.519 | |
Distilled water | 670.00 | ml | - | - | |
1 Adjust pH to 7.5, autoclave at 121°C for 20 min. | |||||
2 For agar plates increase the pH of the Tris/HCl buffer to 8.5. |
1442 | PIRELLULA MEDIUM (M33a) |