Main sol. 1450
Internal Solution-ID: S2982
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 200.0 | g | 200 | 3422.31 | |
| MgSO4 x 7 H2O | 20.0 | g | 20 | 81.144 | |
| KCl | 1.0 | g | 1 | 13.414 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| Casamino acids | 0.5 | g | 0.5 | - | |
| Bacto peptone | 0.5 | g | 0.5 | - | |
| Yeast extract | 0.5 | g | 0.5 | - | |
| Glucose | 0.5 | g | 0.5 | 2.775 | |
|
Starch
(soluble) |
0.5 | g | 0.5 | - | |
| Sodium pyruvate | 0.3 | g | 0.3 | 2.726 | |
| Distilled water | 1000.0 | ml | - | - | |
| Agar , for solid medium | 20.0 | g | 20 | 59.465 | |
| 1 When making media solidified with agar (20 g/l) the agar should not be autoclaved with the NaCl. | |||||
| 2 Adjust to pH 7.2 | |||||
Media with this solution
| 1450 | HALOARCULA ALGERIENSIS MEDIUM |