Main sol. 1456
Internal Solution-ID: S2990
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.5 | 2.459 | |
| Na2SO4 | 2.8 | g | 2.8 | 19.713 | |
|
Yeast extract
(OXOID) |
1.0 | g | 1 | - | |
|
Trypticase peptone
(BD BBL) |
1.0 | g | 1 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.5 | g | 1.5 | 14.153 | |
| Na-pyruvate | 2.5 | g | 2.5 | 22.718 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, pyruvate, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| 1456 | DESULFOSOMA MEDIUM |