Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100 | g | 100 | 1711.16 | |
| Trisodium citrate | 3 | g | 3 | 11.625 | |
| MgSO4 x 7 H2O | 1 | g | 1 | 4.057 | |
| KCl | 2 | g | 2 | 26.828 | |
| Na2CO3 | 3 | g | 3 | 28.305 | |
|
MnCl2 x 4 H2O
(0.36 g/l) |
1 | ml | - | - | |
|
FeSO4 x 7 H2O
(50 g/l) |
1 | ml | - | - | |
|
Yeast extract
(Difco) |
10 | g | 10 | - | |
| Distilled water | 1000 | ml | - | - | |
| Agar , for solid medium | 20 | g | 20 | 59.465 | |
| 1 Final pH 9.0 | |||||
| 2 Add all the components together, except Na2CO3 which is added from a sterilized stock solution when the autoclaved medium has cooled. Agar is added at a concentration of 20 g/l for solid media and should be cooled to about 55°C before the Na2CO3.is added. | |||||
Media with this solution
| 1461 | SALINIVIBRIO SHARMENSIS MEDIUM |