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Main sol. 1473

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Internal Solution-ID: S3018

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.33 g 0.33 2.425
NH4Cl 0.33 g 0.33 6.169
MgCl2 0.33 g 0.33 3.466
CaCl2 0.33 g 0.33 2.974
KCl 0.33 g 0.33 4.427
Trace Element Solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
KNO3 1.00 g 1 9.891
Glucose 2.00 g 2 11.101
Yeast extract
(Sigma) 		0.05 g 0.05 -
Wolin's vitamin solution 1.00 ml - -
1473 PLANTOMYCETACEAE MEDIUM