Main sol. 171
Internal Solution-ID: S302
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
K2HPO4 | 0.22 | g | 0.216 | 1.241 | |
KH2PO4 | 0.22 | g | 0.216 | 1.588 | |
NaH2PO4 x H2O | 4.50 | g | 4.42 | 36.844 | |
Na2HPO4 x 12 H2O | 6.10 | g | 5.992 | 42.21 | |
NH4Cl | 0.31 | g | 0.305 | 5.693 | |
(NH4)2SO4 | 0.22 | g | 0.216 | 1.635 | |
NaCl | 0.45 | g | 0.442 | 7.564 | |
MgSO4 x 7 H2O | 0.09 | g | 0.088 | 0.359 | |
CaCl2 x 2 H2O
(0.1% w/v) |
6.00 | ml | 0.006 | 0.04 | |
FeSO4 x 7 H2O solution (0.1% w/v) | 2.00 | ml | - | - | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.491 | 2.796 | |
Na2S x 9 H2O | 0.50 | g | 0.491 | 2.045 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except cysteine and sulfide, sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.5. | |||||
2 After inoculation pressurize cultivation vessels to one bar overpressure with sterile 80% H2 and 20% CO2 gas mixture. |
171 | THERMOANAEROBACTER KIVUI MEDIUM |