Back to all solutions

Main sol. 171

Internal Solution-ID: S302

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.22 g 0.216 1.241
KH2PO4 0.22 g 0.216 1.588
NaH2PO4 x H2O 4.50 g 4.42 36.844
Na2HPO4 x 12 H2O 6.10 g 5.992 42.21
NH4Cl 0.31 g 0.305 5.693
(NH4)2SO4 0.22 g 0.216 1.635
NaCl 0.45 g 0.442 7.564
MgSO4 x 7 H2O 0.09 g 0.088 0.359
CaCl2 x 2 H2O
(0.1% w/v)
6.00 ml 0.006 0.04
FeSO4 x 7 H2O solution (0.1% w/v) 2.00 ml - -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
L-Cysteine HCl x H2O 0.50 g 0.491 2.796
Na2S x 9 H2O 0.50 g 0.491 2.045
Distilled water 1000.00 ml - -
1 Dissolve ingredients except cysteine and sulfide, sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.5.
2 After inoculation pressurize cultivation vessels to one bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
171 THERMOANAEROBACTER KIVUI MEDIUM