Main sol. 1474
Internal Solution-ID: S3020
Equipment needed: Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.54 | g | 0.54 | 10.095 | |
| KH2PO4 | 0.14 | g | 0.14 | 1.029 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
| Trace element solution | 10.00 | ml | - | - | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Resazurin solution 0.001 g/L | 1.00 | ml | - | - | |
| D-Glucose | 1.80 | g | 1.8 | 9.991 | |
|
Yeast extract
(Difco) |
1.00 | g | 1 | - | |
| NaHCO3 | 2.52 | g | 2.52 | 29.998 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the liquid medium without Vitamin solution, Cysteine-HCl x H2O and Na2S x 9 H2O. Adjust to pH 7.0 with 1 M NaOH. Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v). Sterilize by autoclaving. Before inoculation add from sterile stock solutions Cysteine-HCl x H2O, Vitamin solution (filter sterilized) and Na2S x 9 H2O. | |||||
Media with this solution
| 1474 | PROLIXIBACTER MEDIUM GYSW |