Main sol. 172
Internal Solution-ID: S303
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(Difco) |
1.0 | g | 1 | - | |
|
Tryptone
(Difco) |
1.0 | g | 1 | 14.069 | |
| NaCl | 24.7 | g | 24.7 | 422.656 | |
| KCl | 0.7 | g | 0.7 | 9.39 | |
| MgSO4 x 7 H2O | 6.3 | g | 6.3 | 25.56 | |
| MgCl2 x 6 H2O | 4.6 | g | 4.6 | 22.626 | |
| CaCl2 x 2 H2O | 1.2 | g | 1.2 | 8.162 | |
| NaHCO3 | 0.2 | g | 0.2 | 2.381 | |
|
Agar
(Difco) |
15.0 | g | 15 | 44.599 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.2. Sodium bicarbonate and calcium chloride are autoclaved separately, each in a small volume of distilled water. | |||||
Media with this solution
| 172 | CYTOPHAGA (marine) MEDIUM |